神经母细胞瘤三维体外模型的三维生物打印方案。

Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma.

作者信息

Amoli Mehdi Salar, Rezapourdamanab Sarah, Jin Linqi, Cadena Melissa A, Kaw Kaveeta, Sridhar Vani, Meselhe Mostafa, Krikor Sarah, Mahmoudi Morteza, Ning Liqun, Shim Jenny, Tomov Martin L, Goldsmith Kelly C, Serpooshan Vahid

机构信息

Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Winship Cancer Institute, Emory University, Atlanta, GA, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.

出版信息

STAR Protoc. 2025 Apr 8;6(2):103725. doi: 10.1016/j.xpro.2025.103725.

DOI:10.1016/j.xpro.2025.103725

PMID:40202843

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12008580/

Abstract

Here, we present a protocol for developing an in vitro 3D model of neuroblastoma through bioprinting, incorporating key components of the tumor microenvironment (TME), including vasculature and cancer cell line spheroids. We describe steps for the preparation, bioprinting, and evaluation of tumor models and their use to assess the impact of TME on solid tumor behavior. We report procedures for bioink preparation, bioprinting, the measurement of mechanical properties and fidelity, as well as the viability of different cell types and their interactions. For complete details on the use and execution of this protocol, please refer to Ning et al..

摘要

在此，我们展示了一种通过生物打印开发神经母细胞瘤体外三维模型的方案，该模型纳入了肿瘤微环境（TME）的关键组成部分，包括脉管系统和癌细胞系球体。我们描述了肿瘤模型的制备、生物打印和评估步骤，以及利用它们评估TME对实体瘤行为影响的方法。我们报告了生物墨水制备、生物打印、力学性能和保真度测量，以及不同细胞类型的活力及其相互作用的程序。有关本方案使用和执行的完整详细信息，请参阅宁等人的研究。