Antimicrobial Effects of Capsule on Induced Diarrhea in Mice.

BACKGROUND

capsule (), listed in the Chinese Pharmacopoeia, has been employed for the treatment of infectious diarrhea. Nevertheless, the precise mechanism of remains to be elucidated.

AIM OF THE STUDY

This work was to investigate the antibacterial mode and therapeutic mechanism of towards infected diarrhea.

MATERIALS AND METHODS

HPLC identified the primary chemical constituents of . A model of diarrhea was induced via injection. The impact of on -induced diarrhea was evaluated using a diarrhea score in Babl/c mice. The integrity of the intestinal barrier was assessed through staining and measuring levels of specific proteins. Furthermore, immunofluorescence staining was conducted to identify tight junction proteins in the small intestinal tissue. The full-length 16S rRNA was used to examine gut microbiota. Network pharmacology, molecular docking, and molecular dynamic simulation were used to investigate the impact of on diarrhea-related inflammation and quantify the expression levels of IL-6 and TNF- in the blood and small intestine. The in vivo antibacterial activity and mode of action of were also investigated using IVIS imaging, transmission electron microscopy, scanning electron microscopy, and molecular dynamic simulation.

RESULTS

exhibited a positive effect on infected diarrhea. The content of IL-6 and TNF- in mice with was significantly reduced. Additionally, could restore the intestinal barrier function by reversing small intestine structure and up-regulating the expression of tight junction proteins. The significantly inhibit pathogenic bacteria and restore the gut microbiota diversity. IVIS Imaging System was visually observed significant antibacterial efficacy of in vivo. The disrupted the cell membrane system of through the interaction between bioactive molecule and bilayer.

CONCLUSION

normalized the gut barrier function, reshaped gut microbiota balance, suppressed the inflammatory pathways, and inhibited the bacterial activity, thereby relieving the diarrhea caused by .