The Dawn of High-Throughput and Genome-Scale Kinetic Modeling: Recent Advances and Future Directions.

Researchers have invested much effort into developing kinetic models due to their ability to capture dynamic behaviors, transient states, and regulatory mechanisms of metabolism, providing a detailed and realistic representation of cellular processes. Historically, the requirements for detailed parametrization and significant computational resources created barriers to their development and adoption for high-throughput studies. However, recent advancements, including the integration of machine learning with mechanistic metabolic models, the development of novel kinetic parameter databases, and the use of tailor-made parametrization strategies, are reshaping the field of kinetic modeling. In this Review, we discuss these developments and offer future directions, highlighting the potential of these advances to drive progress in systems and synthetic biology, metabolic engineering, and medical research at an unprecedented scale and pace.