拟南芥中组蛋白H2A与其变体H2A.Z和H2A.W的结构和功能相互关系

Structural and functional interrelationships of histone H2A with its variants H2A.Z and H2A.W in Arabidopsis.

作者信息

Wang Youchao, Wu Jiabing, Yang Shuoming, Li Xiang, Wang Jiachen, Lv Qinghe, Zhu Xiaoyu, Lu Guoliang, Zhang Jinru, Shen Wen-Hui, Liu Bing, Lin Jinzhong, Dong Aiwu

机构信息

State Key Laboratory of Genetics and Development of Complex Phenotypes, Institute of Plant Biology, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, P.R. China.

State Key Laboratory of Genetics and Development of Complex Phenotypes, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, P.R. China; Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, P.R. China; Center for mRNA Translational Research, Fudan University, Shanghai, P.R. China.

出版信息

Structure. 2025 May 6. doi: 10.1016/j.str.2025.04.015.

DOI:10.1016/j.str.2025.04.015

PMID:40359943

Abstract

Multiple histone H2A variants are known in eukaryotes. However, the functional relationship between H2A and its variants in plants remains largely obscure. Using CRISPR-Cas9 editing, we generated a mutant lacking four H2A isoforms in Arabidopsis and analyzed the functional and structural relationships between H2A, H2A.Z, and H2A.W. RNA sequencing and phenotype analyses revealed mild changes in gene transcription and plant development in mutants lacking H2A, H2A.Z, or H2A.W compared with the wild-type plants. Chromatin immunoprecipitation sequencing analysis showed that H2A can substitute for both H2A.Z and H2A.W across the genome, including in euchromatin and heterochromatin regions. However, H2A.Z replaced both H2A and H2A.W primarily within the euchromatin regions. By using DNA and histones from Arabidopsis, we constructed nucleosomes containing H2A, H2A.Z, or H2A.W and resolved their cryogenic electron microscopy (cryo-EM) structures at near-atomic resolution. Collectively, the results reveal the structural similarity and functional redundancy of H2A and its variants in Arabidopsis.

摘要

在真核生物中已知多种组蛋白H2A变体。然而，H2A及其变体在植物中的功能关系在很大程度上仍不清楚。利用CRISPR-Cas9编辑技术，我们在拟南芥中生成了一个缺失四种H2A亚型的突变体，并分析了H2A、H2A.Z和H2A.W之间的功能和结构关系。RNA测序和表型分析显示，与野生型植物相比，缺乏H2A、H2A.Z或H2A.W的突变体在基因转录和植物发育方面发生了轻微变化。染色质免疫沉淀测序分析表明，H2A可以在全基因组范围内替代H2A.Z和H2A.W，包括常染色质和异染色质区域。然而，H2A.Z主要在常染色质区域内替代H2A和H2A.W。通过使用拟南芥的DNA和组蛋白，我们构建了包含H2A、H2A.Z或H2A.W的核小体，并以近原子分辨率解析了它们的低温电子显微镜（cryo-EM）结构。总的来说，这些结果揭示了拟南芥中H2A及其变体的结构相似性和功能冗余性。