Fang Fang, Liu Bin, Chen Qiuzi, Xiao Lisi, Deng Zhuozi, Zhang Zhaoqi, Huang Xuemei, Pang Xuequn
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruit and Vegetables/Engineering Research Center for Postharvest Technology of Horticultural Crops in South China, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2025 Apr 25;26(9):4090. doi: 10.3390/ijms26094090.
Allele-specific expression (ASE) is a phenomenon in which the expression level of an allele from both parents is inconsistent, which is considered to play a key role in the differences between hybrids. As a typical climacteric fruit, banana undergoes a ripening process that affects the quality of the fruit. BaXi (, AAA group) and Fen Jiao (, ABB group) banana fruits show different traits during postharvest ripening, and their high-quality reference genomic sequences have been published. In this work, we analyzed differentially expressed genes (DEGs) in these two banana cultivars based on the transcriptomes during the postharvest stages. Additionally, the imbalance expression of alleles of DEGs in Fen Jiao banana fruit was analyzed, revealing that 27.2% (3 d) and 22.2% (6 d) of the 15,415 DEGs showed ASE. Then, the ASE profiles related to the post-ripening of banana fruit were built, focusing on ripening-related pathways, such as ethylene biosynthesis (62.5-83.3%), starch degradation (0-75%) and cell wall material degradation (34.6-90.9%). The ASE genes involved in ripening were more frequent than those associated with general gene expression. In addition, the candidate key genes of ASE alleles involved in ethylene synthesis and starch degradation were identified, including the alleles of /, /, /, / and / involved in ethylene biosynthesis, and those of /, /, / and / involved in starch degradation. The expression of the B genes of these key enzyme genes (//) is more active than that of the A genes in Fen Jiao bananas.
等位基因特异性表达（ASE）是一种父母双方等位基因表达水平不一致的现象，被认为在杂种差异中起关键作用。香蕉作为典型的跃变型果实，会经历影响果实品质的成熟过程。巴西蕉（AAA组）和粉蕉（ABB组）果实采后成熟过程表现出不同性状，且已公布了它们的高质量参考基因组序列。在这项研究中，我们基于采后阶段的转录组分析了这两个香蕉品种中的差异表达基因（DEG）。此外，还分析了粉蕉果实中DEG等位基因的不平衡表达，结果显示在15415个DEG中，分别有27.2%（3天）和22.2%（6天）表现出ASE。随后，构建了与香蕉果实后熟相关的ASE图谱，重点关注与成熟相关的途径，如乙烯生物合成（62.5 - 83.3%）、淀粉降解（0 - 75%）和细胞壁物质降解（34.6 - 90.9%）。参与成熟的ASE基因比与一般基因表达相关的基因更为常见。此外，还鉴定了参与乙烯合成和淀粉降解的ASE等位基因的候选关键基因，包括参与乙烯生物合成的/、/、/、/和/的等位基因，以及参与淀粉降解的/、/、/和/的等位基因。在粉蕉中，这些关键酶基因（//）的B基因表达比A基因更活跃。
