心房利钠多肽（ANP）：大鼠心房储存高分子量前体，但分泌含25 - 35个氨基酸的加工后肽段。

Atrial natriuretic polypeptides (ANP): rat atria store high molecular weight precursor but secrete processed peptides of 25-35 amino acids.

作者信息

Vuolteenaho O, Arjamaa O, Ling N

出版信息

Biochem Biophys Res Commun. 1985 May 31;129(1):82-8. doi: 10.1016/0006-291x(85)91406-8.

Abstract

A radioimmunoassay was developed for rat atrial natriuretic polypeptides. Using the radioimmunoassay and gel filtration in reducing and dissociating conditions, extracts of rat atria were found to contain mainly a 15000-dalton immunoreactive material, probably corresponding to pronatriodilatin. However, when isolated beating atria were incubated in plasma-free conditions, the secreted immunoreactive material consisted almost exclusively of 2500-3500-dalton peptide(s). These results show that rat atrial cells secrete a low molecular weight natriuretic peptide which is highly active, but store the less active large molecular weight form(s).

摘要

已开发出一种针对大鼠心房利钠多肽的放射免疫分析法。在还原和解离条件下，使用该放射免疫分析法和凝胶过滤法，发现大鼠心房提取物主要含有一种15000道尔顿的免疫反应性物质，可能对应于前心钠素原。然而，当将分离的搏动心房在无血浆条件下孵育时，分泌的免疫反应性物质几乎完全由2500 - 3500道尔顿的肽组成。这些结果表明，大鼠心房细胞分泌一种低分子量的利钠肽，其活性很高，但储存的是活性较低的大分子形式。