用于检测副溶血性弧菌耐热直接溶血素的酶联免疫吸附测定法。
Enzyme-linked immunosorbent assays for detection of thermostable direct hemolysin of Vibrio parahaemolyticus.
作者信息
Honda T, Yoh M, Kongmuang U, Miwatani T
出版信息
J Clin Microbiol. 1985 Sep;22(3):383-6. doi: 10.1128/jcm.22.3.383-386.1985.
Abstract
Several systems for enzyme-linked immunosorbent assay (ELISA) of thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus were tested, and single-antibody sandwich ELISA systems gave satisfactory results. ELISA was able to detect as little as several nanograms of purified TDH per milliliter. The method of De Jong (J. Clin. Microbiol. 17:928-930, 1983) and the glutaraldehyde method were successful for preparing conjugates of alkaline phosphatase and anti-TDH antibody. TDH in fluids in intestinal loops of experimental animals challenged with living V. parahaemolyticus was accurately detectable by ELISA.
摘要
对几种用于副溶血性弧菌耐热直接溶血素（TDH）酶联免疫吸附测定（ELISA）的系统进行了测试，单抗体夹心ELISA系统给出了令人满意的结果。ELISA能够检测到每毫升低至几纳克的纯化TDH。De Jong的方法（《临床微生物学杂志》17:928 - 930，1983年）和戊二醛法成功制备了碱性磷酸酶与抗TDH抗体的结合物。用活的副溶血性弧菌攻击实验动物后，ELISA能准确检测出其肠袢液中的TDH。
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