一项针对绝经前女性的纵向试点研究将宫颈阴道微生物群与CIN3的进展和恢复联系起来。

A longitudinal pilot study in pre-menopausal women links cervicovaginal microbiome to CIN3 progression and recovery.

作者信息

Banila Cristiana, Ladoukakis Efthymios, Scibior-Bentkowska Dorota, Santiago Leandro Rodrigues, Reuter Caroline, Kleeman Michelle, Nedjai Belinda

机构信息

Wolfson Institute of Population Health, Queen Mary University of London, London, UK.

The Institute of Cancer Research (ICR), Genomics Facility, Sutton, UK.

出版信息

Commun Biol. 2025 Jun 6;8(1):883. doi: 10.1038/s42003-025-08328-w.

DOI:10.1038/s42003-025-08328-w

PMID:40481153

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12144234/

Abstract

Increasing evidence suggests vaginal dysbiosis is associated with persistent high-risk human papillomavirus (hrHPV) infection and cervical intraepithelial neoplasia (CIN) development. In this pilot longitudinal study, we investigate the potential of vaginal microbiome biomarkers to predict CIN3 development in hrHPV-positive (hrHPV+) women of reproductive age and assess loop electrosurgical excision procedure (LEEP) outcomes.Fifty-nine non-menopausal women 20-53 years old, with normal cytology, were selected from the ARTISTIC trial and followed up twice over six years. Vaginal microbiome was analysed by 16S rRNA sequencing. HrHPV+ women with CIN3 showed a significant overrepresentation of Sneathia amnii, Megasphaera genomosp., Peptostreptococcus anaerobius and Achromobacter spanius (p < 0.05). Successfully LEEP-treated hrHPV-negative women exhibited increased Lactobacillus species, especially Lactobacillus gasseri. Additionally, Lactobacillus helveticus, suntoryeus and vaginalis showed a potential protective role against CIN3 development.These unique microbial biomarkers associated with CIN3 development and recovery following LEEP treatment bring new insights into the vaginal microbiome's role on disease progression.

摘要

越来越多的证据表明，阴道生态失调与持续性高危型人乳头瘤病毒（hrHPV）感染及宫颈上皮内瘤变（CIN）的发展有关。在这项前瞻性纵向研究中，我们调查了阴道微生物组生物标志物预测育龄期hrHPV阳性（hrHPV+）女性CIN3发展及评估环形电切术（LEEP）结果的潜力。从ARTISTIC试验中选取了59名20-53岁、细胞学检查正常的非绝经女性，并在六年内进行了两次随访。通过16S rRNA测序分析阴道微生物组。CIN3的hrHPV+女性中，羊水斯奈氏菌、基因组巨球形菌、厌氧消化链球菌和西班牙无色杆菌显著增多（p<0.05）。LEEP治疗成功的hrHPV阴性女性中，乳酸杆菌种类增加，尤其是加氏乳酸杆菌。此外，瑞士乳酸杆菌、三得利乳酸杆菌和阴道乳酸杆菌对CIN3发展显示出潜在的保护作用。这些与CIN3发展及LEEP治疗后恢复相关的独特微生物生物标志物为阴道微生物组在疾病进展中的作用带来了新见解。