Kosakai M, Isemura M, Munakata H, Yosizawa Z
Tohoku J Exp Med. 1985 Jul;146(3):303-11. doi: 10.1620/tjem.146.303.
The activities of six glycosidases in a rat colorectal adenocarcinoma were measured and compared with those of normal colonic mucosa. The specific activities of beta-galactosidase (EC 3.2.1.23) and beta-glucuronidase (EC 3.2.1.31) in the adenocarcinoma were similar to those of the corresponding ones in the normal mucosa, whereas those of beta-N-acetylglucosaminidase (EC 3.2.1.30), alpha-L-fucosidase (EC 3.2.1.51), alpha-galactosidase (EC 3.2.1.22) and beta-glucosidase (EC 3.2.1.21) were reduced in the former as compared with those in the latter. In the case of alpha-L-fucosidase, two forms were newly detected in the tumor. The relative abundance of three forms of beta-N-acetylglucosaminidase was quite different between the adenocarcinoma and the normal mucosa, and the level of the intermediate form in the tumor was markedly reduced. However, thermostability and Km values of two forms A and B in the tumor were not different from those of the corresponding ones in the normal tissue.
测定了大鼠结肠腺癌中六种糖苷酶的活性，并与正常结肠黏膜中的活性进行了比较。腺癌中β-半乳糖苷酶（EC 3.2.1.23）和β-葡萄糖醛酸酶（EC 3.2.1.31）的比活性与正常黏膜中相应酶的比活性相似，而β-N-乙酰氨基葡萄糖苷酶（EC 3.2.1.30）、α-L-岩藻糖苷酶（EC 3.2.1.51）、α-半乳糖苷酶（EC 3.2.1.22）和β-葡萄糖苷酶（EC 3.2.1.21）在腺癌中的活性相对于正常黏膜有所降低。就α-L-岩藻糖苷酶而言，在肿瘤中新检测到两种形式。腺癌和正常黏膜中三种形式的β-N-乙酰氨基葡萄糖苷酶的相对丰度差异很大，肿瘤中中间形式的水平明显降低。然而，肿瘤中两种A和B形式的热稳定性和Km值与正常组织中相应形式的热稳定性和Km值并无差异。
