新型隐球菌蛋白质组胰蛋白酶消化的时间和温度优化
Temporal and thermal optimization of trypsin digestion for the cryptococcal proteome.
作者信息
Sun Jing, McAlister Jason A, Geddes-McAlister Jennifer
机构信息
Molecular and Cellular Biology Department, University of Guelph, Guelph, Ontario, Canada.
出版信息
Microbiol Resour Announc. 2025 Jul 10;14(7):e0113724. doi: 10.1128/mra.01137-24. Epub 2025 Jun 11.
Abstract
Proteomics investigates diverse biological systems, providing insights into regulation, signaling, modifications, and interactions. Optimized sample preparation is critical to ensure robust and reproducible analyses balanced with time and cost. Herein, we evaluated trypsin digestion parameters for the human fungal pathogen, , and determined conditions for optimal protein identification.
摘要
蛋白质组学研究多种生物系统，为调控、信号传导、修饰和相互作用提供见解。优化的样品制备对于确保在时间和成本之间取得平衡的稳健且可重复的分析至关重要。在此，我们评估了人类真菌病原体的胰蛋白酶消化参数，并确定了最佳蛋白质鉴定的条件。
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