Suyama Keitaro, Nose Takeru
Faculty of Arts and Science, Kyushu University, Fukuoka, Japan.
Department of Chemistry, Faculty and Graduate School of Science, Kyushu University, Fukuoka, Japan.
Methods Mol Biol. 2025;2931:91-103. doi: 10.1007/978-1-0716-4562-8_9.
Elastin-like peptides (ELPs) are synthetic, repetitive sequences known for their temperature-dependent reversible self-assembly, making them environmentally friendly, biocompatible, and thermoresponsive. Despite various strategies for ELP production, the development of effective ELP supply methods remains a challenge. This chapter outlines a practical approach to synthesizing thermoresponsive ELPs using liquid-phase peptide synthesis (LPPS) with a hydrophobic benzyl alcohol support (HBA-Tag) and fragment condensation. This method effectively produces short ELPs that exhibit temperature-responsive self-assembly.
弹性蛋白样肽（ELPs）是合成的重复序列，以其温度依赖性可逆自组装而闻名，使其具有环境友好、生物相容性和热响应性。尽管有多种ELP生产策略，但开发有效的ELP供应方法仍然是一个挑战。本章概述了一种使用带有疏水苄醇支持物（HBA-Tag）的液相肽合成（LPPS）和片段缩合来合成热响应性ELPs的实用方法。该方法有效地产生了表现出温度响应自组装的短ELPs。
