用于天然产物发现的全局转录调节因子（GTRs）工程。
Engineering of Global Transcriptional Regulators (GTRs) in for Natural Product Discovery.
作者信息
Zhao Yujie, Gong Qing, Zhang Huawei
机构信息
School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, China.
出版信息
J Fungi (Basel). 2025 Jun 12;11(6):449. doi: 10.3390/jof11060449.
The genus is an important group of filamentous fungi, and the various biological activities of its secondary metabolites (SMs) have great biosynthetic potential. Despite over 4200 SMs having been isolated from spp., their metabolic potential remains unexplored due to the presence of numerous silent biosynthetic gene clusters (BGCs) in their genomes. Fortunately, over the last two decades, the global transcriptional regulator (GTR) engineering strategy has emerged as a powerful tool for activating these cryptic BGCs in order to synthesize previously undiscovered SMs from spp. This review highlights recent advances in fungal GTR engineering techniques, the regulatory mechanisms of GTRs, and current challenges and future perspectives for their application in natural product discovery in the genus .
该属是丝状真菌的一个重要类群，其次级代谢产物（SMs）的各种生物活性具有巨大的生物合成潜力。尽管已从该属物种中分离出4200多种SMs，但由于其基因组中存在大量沉默的生物合成基因簇（BGCs），它们的代谢潜力仍未得到探索。幸运的是，在过去二十年中，全球转录调节因子（GTR）工程策略已成为激活这些隐秘BGCs的有力工具，以便从该属物种中合成以前未发现的SMs。本综述重点介绍了真菌GTR工程技术的最新进展、GTRs的调控机制，以及它们在该属天然产物发现中的应用面临的当前挑战和未来前景。
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