基于全基因组关联研究分析鉴定江西地方品种睾丸性状相关新基因座
Identification of Novel Genetic Loci Involved in Testis Traits of the Jiangxi Local Breed Based on GWAS Analyses.
作者信息
Ma Jing-E, Huang Ke, Gibril Bahareldin Ali Abdalla, Xiong Xinwei, Wu Yanping, Wang Zhangfeng, Xu Jiguo
机构信息
Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang 330032, China.
Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China.
出版信息
Genes (Basel). 2025 May 27;16(6):637. doi: 10.3390/genes16060637.
BACKGROUND
The testis, a critical reproductive organ in male animals, is responsible for sperm production and androgen secretion. Testis weight often correlates with reproductive performance, yet the genetic factors influencing testicular traits in chickens remain unclear.
METHODS
Previous genome-wide association studies (GWAS) have identified key genes affecting testicular traits in Kangle Yellow chickens, along with the associated regulatory pathways and Gene Ontology (GO) terms, through bioinformatic analyses. In this study, we utilized the existing literature, full-length transcriptome data, and proteome analyses to select key candidate genes.
RESULTS
We identified 13 associated markers for chicken testicular traits with 262 candidate genes. Nine candidate genes were found to regulate chicken testicular traits referred to integrated analysis, including , , , , , , , , and . Physical interactions among these genes were also observed, implicating mechanisms such as cell adhesion molecules and neuroactive ligand-receptor interaction.
CONCLUSIONS
These findings provide a genetic basis for improving testicular traits in Chinese native chicken breeds.
背景
睾丸是雄性动物重要的生殖器官，负责精子生成和雄激素分泌。睾丸重量通常与繁殖性能相关，但影响鸡睾丸性状的遗传因素仍不清楚。
方法
以往的全基因组关联研究（GWAS）通过生物信息学分析，在康乐黄鸡中鉴定出影响睾丸性状的关键基因，以及相关的调控途径和基因本体（GO）术语。在本研究中，我们利用现有文献、全长转录组数据和蛋白质组分析来选择关键候选基因。
结果
我们鉴定出13个与鸡睾丸性状相关的标记以及262个候选基因。综合分析发现9个候选基因调控鸡睾丸性状，包括 、 、 、 、 、 、 、 和 。还观察到这些基因之间存在物理相互作用，涉及细胞粘附分子和神经活性配体-受体相互作用等机制。
结论
这些发现为改善中国本土鸡品种的睾丸性状提供了遗传基础。
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