Araujo José Rafael da Silva, de Felício Rafael, Marinho da Silva Camila, Oliveira Palloma Lima de, Araújo Silvany de Sousa, Sommaggio Laís Roberta Deroldo, da Silva Adriana Fabiana Corrêa, Nunes Paulo Henrique Valença, Veras Bruno Oliveira de, de Oliveira Erwelly Barros, Aguiar Jaciana Dos Santos, Marin-Morales Maria Aparecida, Trivella Daniela Barretto Barbosa, Benko-Iseppon Ana Maria, Silva Márcia Vanusa da, Brasileiro-Vidal Ana Christina
Departamento de Genética, Universidade Federal de Pernambuco, Recife 50670-901, Brazil.
Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas 13083-100, Brazil.
Pharmaceuticals (Basel). 2025 Jun 10;18(6):863. doi: 10.3390/ph18060863.
has long been used in Latin American folk medicine for the treatment of respiratory and gastrointestinal disorders. Therefore, toxicological and phytochemical investigations are required to assess the safety and support the evidence-based use of its bark in medicinal applications. This study aimed to evaluate the aqueous bark extract of , focusing on its chemical composition and its antioxidant, cytotoxic, and genotoxic properties. : The aqueous extract was obtained by decoction of dried bark samples. Phytochemical characterization was conducted using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS), and data were processed using the NP MS Workflow 1.1.4 software, allowing for the annotation of key secondary metabolites. Antioxidant activity was assessed through multiple in vitro assays, including DPPH, ABTS, phosphomolybdenum, and reducing power tests. Cytotoxicity was evaluated using the MTT assay, while genotoxicity was investigated through the Ames test and micronucleus assay. : Phytochemical analysis revealed several flavonoids, with procyanidin B2 annotated as a major compound. The extract exhibited strong antioxidant activity, with EC values of 5.43 μg/mL (DPPH), 12.40 μg/mL (ABTS), 35.20 μg/mL (phosphomolybdenum), and 31.27 μg/mL (reducing power). The MTT assay showed no cytotoxic effects at concentrations up to 6400 μg/mL. Furthermore, both the Ames and micronucleus assays showed the absence of genotoxic effects at concentrations up to 1600 μg/plate and 400 μg/mL, respectively. : The aqueous bark extract of demonstrates strong antioxidant potential and a favorable safety profile, with no detectable cytotoxicity or genotoxicity at concentrations effective in antioxidant assays. Further studies are recommended to confirm and validate its traditional medicinal properties using appropriate in vivo models, followed by pre-clinical evaluations.
长期以来，它一直被用于拉丁美洲民间医学中治疗呼吸道和胃肠道疾病。因此，需要进行毒理学和植物化学研究，以评估其安全性并为其树皮在药用中的循证使用提供支持。本研究旨在评估[植物名称]的树皮水提取物，重点关注其化学成分及其抗氧化、细胞毒性和遗传毒性特性。方法：通过煎煮干燥的树皮样品获得水提取物。使用超高效液相色谱-串联质谱法（UPLC-MS/MS）进行植物化学表征，并使用NP MS Workflow 1.1.4软件处理数据，以注释关键次生代谢物。通过多种体外试验评估抗氧化活性，包括DPPH、ABTS、磷钼酸和还原力测试。使用MTT试验评估细胞毒性，同时通过Ames试验和微核试验研究遗传毒性。结果：植物化学分析揭示了几种黄酮类化合物，原花青素B2被注释为主要化合物。该提取物表现出较强的抗氧化活性，DPPH的EC值为5.43μg/mL，ABTS的EC值为12.40μg/mL，磷钼酸的EC值为35.20μg/mL，还原力的EC值为31.27μg/mL。MTT试验表明，在浓度高达6400μg/mL时没有细胞毒性作用。此外，Ames试验和微核试验均表明，在浓度分别高达1600μg/平板和400μg/mL时没有遗传毒性作用。结论：[植物名称]的树皮水提取物具有较强的抗氧化潜力和良好的安全性，在抗氧化试验有效浓度下未检测到细胞毒性或遗传毒性。建议进一步研究，使用适当的体内模型确认和验证其传统药用特性，随后进行临床前评估。
