BACKGROUND

Amide serves as the key functional group for several chemical families, such as proteins, peptides, amides, and amino acid-conjugated bile acids (AA-BAs), and the featured fragment ions (e.g., a, b, c, x, y, and z) are usually the determinant clues for MS/MS-dependent structural analysis. Here, efforts were made to identify the fragment ions through matching full exciting energy ramp (FEER)-MS spectra with authentic amines and/or known substructures, and the justified strategy was thereafter applied to profile AA-BAs-targeted sub-metabolome in biological matrices.

RESULTS

Forty-six amides were collected to configure FEER-MS for the featured fragment ions, and FEER-MS spectra were acquired for some relevant amines. When amides owned identical substructure, they generated the same FEER-MS spectra correlating to the building blocks, and FEER-MS spectrum of y ion for a given amide matched exactly with FEER-MS of the pioneer amine. To assist structural analysis of AA-BAs, 112 authentic compounds were obtained by integrating commercial collection, synthesis, and biotransformation. Through matching FEER-MS spectra with FEER-MS of known substructures or FEER-MS of authentic amines, 85 AA-BAs were identified from a pooled biological sample. Quantitative sub-metabolome comparison involving all detected AA-BAs was conducted between healthy and CCl-induced liver injury rats. Ala-CA, Leu-CA, Trp-CA, Tyr-CA, Phe-CA, together with Ile-UCA isomer served as the primary differential AA-BA species.

SIGNIFICANCE AND NOVELTY

Differential AA-BAs bearing confirmative identities were disclosed to discriminate liver injury rats from healthy animals. More importantly, a meaningful bottom-up structural analysis strategy that deciphers the featured fragment ions via matching FEER-MS (n = 2 or 3) with authentic pioneers or known substructures was constructed for amides as well as other chemical families.