新型隐球菌与小胶质细胞的体外相互作用作为研究中枢神经系统感染的工具

Cryptococcus neoformans and Microglia In Vitro Interaction as a Tool to Study Central Nervous System Infection.

作者信息

Albuquerque Patrícia, Nicola André Moraes, Paes Hugo Costa

机构信息

Faculty of Health Sciences and Technologies, University of Brasília, Brasília, DF, Brazil.

The Jill Roberts Institute for Research in Inflammatory Bowel Disease, Weill Cornell Medicine, Cornell University, New York, NY, USA.

出版信息

Methods Mol Biol. 2025;2950:167-178. doi: 10.1007/978-1-0716-4674-8_11.

DOI:10.1007/978-1-0716-4674-8_11

PMID:40622534

Abstract

This chapter presents in vitro protocols for studying interactions between Cryptococcus neoformans and microglial cells. These phagocytes are a critical component of the central nervous system's immune response to C. neoformans, the most common fungal agent of meningoencephalitis worldwide. Given its severity and high incidence in immunocompromised patients, understanding the interaction of C. neoformans with central nervous system (CNS) cells could help advance treatment strategies. These protocols leverage microscopy and molecular biology techniques to elucidate the mechanisms of C. neoformans invasion and persistence within the CNS, offering a methodological framework for fungal neuropathogenesis research.

摘要

本章介绍了用于研究新型隐球菌与小胶质细胞之间相互作用的体外实验方案。这些吞噬细胞是中枢神经系统对新型隐球菌免疫反应的关键组成部分，新型隐球菌是全球最常见的脑膜脑炎真菌病原体。鉴于其在免疫功能低下患者中的严重性和高发病率，了解新型隐球菌与中枢神经系统（CNS）细胞的相互作用有助于推进治疗策略。这些实验方案利用显微镜和分子生物学技术来阐明新型隐球菌在中枢神经系统内的侵袭和持续存在机制，为真菌神经病理学研究提供了一个方法框架。