胆管癌成年患者的肠道微生物群可识别失调的布劳特氏菌属物种和胆汁酸代谢途径。

Intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated Blautia species and bile acid metabolic pathways.

作者信息

De Tianyu, Ma Tian, Wang Wei, An Xusheng, Liu Di, Yin Hongkun, Wang Qi, Zhao Ting, Wang Hao

机构信息

General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China.

Peking University First Hospital Ningxia Women and Children's Hospital, No. 127 Hupan Street, Jinfeng District, Yinchuan, Ningxia Hui, China.

出版信息

BMC Gastroenterol. 2025 Jul 7;25(1):506. doi: 10.1186/s12876-025-04096-3.

DOI:10.1186/s12876-025-04096-3

PMID:40624612

Abstract

BACKGROUND

Cholangiocarcinoma (CCA) represents a significant global health concern. The gut and bile microbiota, which can influence the gut-liver axis and disease progression, have not been thoroughly characterized in CCA patients.

METHODS

We selected two clinical centers at our hospital and collected stool samples from CCA patients and healthy controls (HC). These samples underwent whole-genome metagenomic shotgun sequencing, followed by analysis using both marker gene-based and assembly-based methods. Additionally, KEGG pathway enrichment was performed using the cholangiocarcinoma (CHOL) RNA-seq samples.

RESULTS

Our results revealed distinct dysbiosis of the gut microbiota in our regional CCA patients. The results revealed greater heterogeneity in the gut microbiome of CCA patients compared to HC samples. We found Blautia species to be significantly less abundant in CCA samples, and can distinguish CCA patients from HC. Blautia can also play a role in influencing the modification of secondary bile acids. Additionally, down-regulation of arachidonic acid and linoleic acid metabolism was observed in the tumor tissues of CHOL patients. In summary, the results revealed significant heterogeneity difference in the gut microbiome of CCA patients compared to HC samples, and detected the specifically decreased Blautia species in CCA patients, suggesting that Blautia may influence bile acid metabolic pathways. Further investigation is warranted to explore Blautia as a potential biomarker for CCA.

摘要

背景

胆管癌（CCA）是一个重大的全球健康问题。肠道和胆汁微生物群可影响肠-肝轴及疾病进展，但在CCA患者中尚未得到充分表征。

方法

我们选择了我院的两个临床中心，收集了CCA患者和健康对照（HC）的粪便样本。这些样本进行了全基因组宏基因组鸟枪法测序，随后使用基于标记基因和基于组装的方法进行分析。此外，使用胆管癌（CHOL）RNA测序样本进行KEGG通路富集分析。

结果

我们的结果显示，本地区CCA患者的肠道微生物群存在明显的生态失调。结果显示，与HC样本相比，CCA患者的肠道微生物组具有更大的异质性。我们发现CCA样本中布劳特氏菌属的丰度显著降低，并且可以区分CCA患者和HC。布劳特氏菌属还可在影响次级胆汁酸修饰方面发挥作用。此外，在CHOL患者的肿瘤组织中观察到花生四烯酸和亚油酸代谢下调。总之，结果显示与HC样本相比，CCA患者的肠道微生物组存在显著的异质性差异，并检测到CCA患者中布劳特氏菌属特异性减少，提示布劳特氏菌属可能影响胆汁酸代谢途径。有必要进一步研究以探索布劳特氏菌属作为CCA潜在生物标志物的可能性。