使用金属有机框架聚多巴胺纳米颗粒增强和协同递送RNA干扰用于生物农药应用。
Increased and synergistic RNAi delivery using MOF polydopamine nanoparticles for biopesticide applications.
作者信息
Gao Zhou, Rensing Christopher, Wang Jie, Shen Chenhui, Elzaki Mohammed Esmail Abdalla, Li Xiaoyun, Tan Jinfang, Jiang Xiaoqian
机构信息
School of Agriculture and Biotechnology, Sun Yat-sen University, Shenzhen, PR China.
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, PR China.
出版信息
Nat Commun. 2025 Jul 10;16(1):6384. doi: 10.1038/s41467-025-61604-5.
RNA interference is an eco-friendly alternative to chemical pesticides, yet its efficacy in lepidopterans like Spodoptera frugiperda (S. frugiperda) is limited by poor uptake. Here, we report on ZIF-8 polydopamine nanoparticles that protect dsRNA against enzymatic degradation and active the endocytic/phagosome pathways for increased uptake. Furthermore, the uptake of nano-enabled dsRNA induces the overgrowth of Serratia marcescens, this reduces the S. frugiperda reactive oxygen species (ROS) immune response, increasing the effects of plant's natural defenses, further inhibiting Enteroccous mundtii growth. This work shows the synergistic potential of nanoparticles for influencing the gut bacteria to prevent resistance mechanisms and for RNAi delivery for pest management.
RNA干扰是一种对环境友好的化学杀虫剂替代品，但其在诸如草地贪夜蛾等鳞翅目昆虫中的功效因摄取不良而受到限制。在此，我们报道了ZIF-8聚多巴胺纳米颗粒，其可保护双链RNA免受酶降解，并激活内吞/吞噬体途径以增加摄取。此外，纳米化双链RNA的摄取会诱导粘质沙雷氏菌过度生长，这会降低草地贪夜蛾的活性氧（ROS）免疫反应，增强植物天然防御的效果，进一步抑制蒙氏肠球菌的生长。这项工作展示了纳米颗粒在影响肠道细菌以预防抗性机制以及用于害虫管理的RNA干扰递送方面的协同潜力。
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