给予NeuroAid（MLC 901）后，创伤性脑损伤大鼠体内肿瘤坏死因子-α水平升高。
Increased levels of tumor necrosis factor-alpha in rat with traumatic brain injury after NeuroAid (MLC 901) administration.
作者信息
Priyanto Bambang, Islam Andi Asadul, Hatta Mochammad, Bukhari Agussalim, Eka Putra I Wayan Gede Artawan, Abdurrosid Lalu Muhammad, Prihastomo Krisna Tsaniadi
机构信息
Department of Neurosurgery, Medical Faculty of Mataram University, West Nusa Tenggara General Hospital, Mataram, Indonesia.
Department of Neurosurgery, Medical Faculty of Mataram University, Mataram City, West Nusa Tenggara, Indonesia.
出版信息
Surg Neurol Int. 2025 Jun 27;16:259. doi: 10.25259/SNI_301_2025. eCollection 2025.
BACKGROUND
Tumor necrosis factor-alpha (TNF-α) is an inflammatory cytokine produced by macrophages in acute inflammatory processes and plays roles in cell signaling that cause necrosis and apoptosis. This study aimed to show whether there was an effect of Neuroaid (MLC 901) on TNF-α levels in rats with traumatic brain injury (TBI) measured using the Enzyme-linked immunosorbent assay in the peripheral blood.
METHODS
A total of 10 Sprague-Dawley rats were divided into two groups, one group was given MLC 901 ( = 5), and the other group was not given MLC 901 (NaCl 0.9%) ( = 5). All groups were treated with brain injury using the modified Marmarou model. The measurements of TNF-α were performed at 30 min and 6 weeks after brain injury.
RESULTS
At 30 min after brain injury, the TNF-α level in the MLC 901 group was higher (3564.8) than the 0.9% NaCl group (3453.6), but it was not statistically significant ( = 0.830). At 6 weeks of treatment, the TNF-α level in the MLC 901 group (2576.6) was higher than the 0.9% NaCl group (1383.4) and statistically significant ( = 0.001). This study showed that the administration of MLC 901 could increase TNF-α levels at 6 weeks after treatment.
CONCLUSION
MLC 901 increases TNF-α levels in rats with TBI, with a significant rise observed at 6 weeks, suggesting a sustained inflammatory response.
背景
肿瘤坏死因子-α（TNF-α）是巨噬细胞在急性炎症过程中产生的一种炎性细胞因子，在导致坏死和凋亡的细胞信号传导中发挥作用。本研究旨在探讨脑得生（MLC 901）对创伤性脑损伤（TBI）大鼠外周血中TNF-α水平的影响，采用酶联免疫吸附测定法进行检测。
方法
将10只Sprague-Dawley大鼠分为两组，一组给予MLC 901（n = 5），另一组不给予MLC 901（0.9%氯化钠）（n = 5）。所有组均采用改良的Marmarou模型进行脑损伤处理。在脑损伤后30分钟和6周时测量TNF-α。
结果
脑损伤后30分钟，MLC 901组的TNF-α水平（3564.8）高于0.9%氯化钠组（3453.6），但差异无统计学意义（P = 0.830）。治疗6周时，MLC 901组的TNF-α水平（2576.6）高于0.9%氯化钠组（1383.4），差异有统计学意义（P = 0.001）。本研究表明，给予MLC 901可使治疗6周后的TNF-α水平升高。
结论
MLC 901可使TBI大鼠的TNF-α水平升高，在6周时显著升高，提示存在持续的炎症反应。
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