指状青霉中一种新型线粒体病毒的全基因组分析

Complete genome analysis of a novel mitovirus in the phytopathogenic fungus Penicillium digitatum.

作者信息

Tang Zhimin, He Yunhui, Yang Ya, Zhang Xiaoping, Zhou Rui, Zhou Kang

机构信息

Chenzhou Institute of Agricultural Science, Chenzhou, 423000, China.

Chenzhou Branch, Hunan Academy of Agricultural Sciences, Chenzhou, 423000, China.

出版信息

Arch Virol. 2025 Jul 17;170(8):182. doi: 10.1007/s00705-025-06364-9.

DOI:10.1007/s00705-025-06364-9

PMID:40673953

Abstract

Citrus is one of the most widely cultivated and economically important fruit crops globally. However, the postharvest pathogen Penicillium digitatum causes green mold decay in citrus fruits, leading to significant economic losses. Here, a novel mycovirus, Penicillium digitatum mitovirus 1 (PdMV1), was isolated from the phytopathogenic fungus P. digitatum, and its molecular characteristics were determined. PdMV1 has a genome that is 2,517 nt in length with an A+U content of 62.7%. The single ORF encodes a putative RNA-dependent RNA polymerase (RdRp) of 734 amino acids with a molecular mass of 84.116 kDa. A BLASTp comparison of the RdRp sequence showed the highest similarity (65.01% identity) to that of Sclerotinia sclerotiorum mitovirus 32. Phylogenetic analysis revealed that PdMV1 is a new member of the genus Unuamitovirus within the family Mitoviridae. To the best of our knowledge, this is the first report of a mitovirus infecting Penicillium digitatum.

摘要

柑橘是全球种植最广泛且经济上最重要的水果作物之一。然而，采后病原菌指状青霉会导致柑橘类水果发生绿霉腐烂，造成重大经济损失。在此，从植物病原真菌指状青霉中分离出一种新型的真菌病毒——指状青霉线粒体病毒1（PdMV1），并确定了其分子特征。PdMV1基因组长度为2517个核苷酸，A+U含量为62.7%。单一开放阅读框编码一个推定的RNA依赖RNA聚合酶（RdRp），由734个氨基酸组成，分子量为84.116 kDa。RdRp序列的BLASTp比较显示，其与核盘菌线粒体病毒32的序列相似度最高（同一性为65.01%）。系统发育分析表明，PdMV1是线粒体病毒科Unuamitovirus属的一个新成员。据我们所知，这是关于感染指状青霉的线粒体病毒的首次报道。