在精液稀释液中添加肉桂醛以提高卡吉里公羊精液解冻后的精子质量。

The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post-Thaw Sperm Quality of Kajli Ram Semen.

作者信息

Qadeer Saima, Ashraf Asma, Perveen Naila, Asad Muhammad, Husna Asma Ul, Azam Asima, Farooq Muhammad Umer, Alhomida Abdullah S, Khan Haseeb A

机构信息

Department of Zoology, Division of Science and Technology, University of Education, Lahore, Pakistan.

Department of Biology, University of Haripur, Haripur, Pakistan.

出版信息

Vet Med Sci. 2025 Jul;11(4):e70484. doi: 10.1002/vms3.70484.

DOI:10.1002/vms3.70484

PMID:40677171

Abstract

Cinnamaldehyde, a component of cinnamon bark extract, acts as a potential antioxidant by enhancing the activities of catalase, superoxide dismutase and glutathione peroxidase in various cell and animal models. This study aimed to evaluate the protective effects of cinnamaldehyde on the quality of Kajli ram sperm at 4°C and after freezing. Semen samples were obtained from 12 Kajli rams through an artificial vagina maintained at 42°C. Each ejaculate was divided into five aliquots and extended in a tris-citric acid extender with different concentrations of cinnamaldehyde (0%, 0.5%, 1.0%, 2.5% and 5.0%). Post-thaw semen quality was assessed. These results showed that the extender containing 0.5% cinnamaldehyde exhibited significant protective effects (p < 0.05) on post-thaw sperm plasma membrane and sperm viability compared to the control and other experimental groups. In conclusion, supplementing the extender with 0.5% cinnamaldehyde effectively preserved the quality of post-thawed sperm from Kajli rams.

摘要

肉桂醛是肉桂树皮提取物的一种成分，在各种细胞和动物模型中，它通过增强过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性，发挥潜在的抗氧化作用。本研究旨在评估肉桂醛对4℃及冷冻后Kajli公羊精子质量的保护作用。通过保持在42℃的人工阴道从12只Kajli公羊采集精液样本。每次射精分为五等份，并用含有不同浓度肉桂醛（0%、0.5%、1.0%、2.5%和5.0%）的三羟甲基氨基甲烷 - 柠檬酸稀释液进行稀释。评估解冻后精液质量。这些结果表明，与对照组和其他实验组相比，含有0.5%肉桂醛的稀释液对解冻后精子质膜和精子活力具有显著的保护作用（p < 0.05）。总之，在稀释液中添加0.5%肉桂醛可有效保存Kajli公羊解冻后精子的质量。