具有再矿化能力的pH响应性介孔二氧化硅纳米颗粒增强复合树脂。
pH-Responsive mesoporous silica nanoparticle-reinforced composite resin with remineralization capability.
作者信息
Ge Yongcheng, Zhao Ting, Liu Yuguang, Fan Sizheng, Liu Pengyuan, Zhao Chengji, Liu Xiaoqiu
机构信息
Department of Geriatric Stomatology, Hospital of Stomatology, Jilin University, Changchun, 130026, China.
Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, 130021, China.
出版信息
BMC Oral Health. 2025 Jul 23;25(1):1234. doi: 10.1186/s12903-025-06471-8.
OBJECTIVE
Enhancing the mechanical properties of composite resins and promoting dentin remineralization are effective strategies for extending the longevity of restorations. In this study, we developed a pH-responsive composite resin reinforced with phosphorylated chitosan (Pchi)/amorphous calcium phosphate (ACP) and amino-functionalized mesoporous silica nanoparticles (MSNs) (Pchi/ACP@A-MSN complexes).
METHODS
MSNs and Pchi/ACP were synthesized, and the resulting composite resins were classified into five groups based on the filler compositions: Group A (45wt.% A-MSNs + 15wt.% Pchi/ACP + 40wt.% resin matrix), Group B (22.5wt.% A-MSNs + 37.5wt.% Pchi/ACP@A-MSNs + 40wt.% resin matrix), Group C (45wt.% nonporous silica + 15wt.% Pchi/ACP + 40wt.% resin matrix), Group D (15wt.% Pchi/ACP + 85wt.% resin matrix), and Group E (100wt.% resin matrix). The degree of double-bond conversion and mechanical properties (Flexural modulus, flexural strength, and Vickers hardness) of each group were measured. The remineralization performance and calcium ion (Ca²⁺) release performance were observed. Cell viability was evaluated by CCK-8 assay.
RESULTS
Group A exhibited the highest mechanical performance, with flexural strength of 115 ± 9.68 MPa, flexural modulus of 5.99 ± 0.44 GPa, and Vickers hardness of 31.73 ± 2.39 HV. All groups achieved double-bond conversion rates > 50% after 60 s of light curing (P > 0.05). At pH 4.0, Group B exhibited the highest Ca²⁺ release (276 µg/mL), while Group D had the highest release at pH 7.4 (128 µg/mL). SEM and EDS analyses indicated that Group D achieved the most substantial dentin remineralization, followed by Groups C and A. All groups maintained cell viability above 75% throughout the 7-day assay, with no significant cytotoxicity observed.
CONCLUSION
A composite formulation containing 45 wt% A-MSNs and 15 wt% Pchi/ACP (Group A) significantly enhanced mechanical strength. The 15 wt% Pchi/ACP formulation (Group D) demonstrated the greatest remineralization effect. Group B, combining A-MSNs and Pchi/ACP@A-MSNs, provided pH-responsive Ca²⁺ release with a favorable balance of mechanical performance and remineralization potential. All formulations met the biocompatibility requirements for dental applications.
目的
增强复合树脂的机械性能并促进牙本质再矿化是延长修复体使用寿命的有效策略。在本研究中，我们开发了一种用磷酸化壳聚糖（Pchi）/无定形磷酸钙（ACP）和氨基功能化介孔二氧化硅纳米颗粒（MSNs）增强的pH响应性复合树脂（Pchi/ACP@A-MSN复合物）。
方法
合成了MSNs和Pchi/ACP，并根据填料组成将所得复合树脂分为五组：A组（45wt.% A-MSNs + 15wt.% Pchi/ACP + 40wt.%树脂基质），B组（22.5wt.% A-MSNs + 37.5wt.% Pchi/ACP@A-MSNs + 40wt.%树脂基质），C组（45wt.%无孔二氧化硅 + 15wt.% Pchi/ACP + 40wt.%树脂基质），D组（15wt.% Pchi/ACP + 85wt.%树脂基质），E组（100wt.%树脂基质）。测量了每组的双键转化率和机械性能（弯曲模量、弯曲强度和维氏硬度）。观察了再矿化性能和钙离子（Ca²⁺）释放性能。通过CCK-8测定法评估细胞活力。
结果
A组表现出最高的机械性能，弯曲强度为115±9.68MPa，弯曲模量为5.99±0.44GPa，维氏硬度为31.73±2.39HV。所有组在光固化60秒后双键转化率均>50%（P>0.05）。在pH 4.0时，B组Ca²⁺释放量最高（276μg/mL），而在pH 7.4时，D组释放量最高（128μg/mL）。扫描电子显微镜（SEM）和能谱分析（EDS）表明，D组实现了最显著的牙本质再矿化，其次是C组和A组。在整个7天的试验中，所有组的细胞活力均保持在75%以上，未观察到明显的细胞毒性。
结论
含有45 wt% A-MSNs和15 wt% Pchi/ACP的复合配方（A组）显著提高了机械强度。15 wt% Pchi/ACP配方（D组）表现出最大的再矿化效果。结合A-MSNs和Pchi/ACP@A-MSNs的B组提供了pH响应性Ca²⁺释放，在机械性能和再矿化潜力之间取得了良好的平衡。所有配方均满足牙科应用的生物相容性要求。
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