Shen Yu-Ping, Kokaia Zaal
Laboratory of Stem Cells and Restorative Neurology, Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
Department of Physical Medicine and Rehabilitation, Tri-Service General Hospital, School of Medicine, National Defense Medical Center, Taipei 114, Taiwan.
Cells. 2025 Jul 14;14(14):1074. doi: 10.3390/cells14141074.
Brain organoid technology has seen significant development in recent years. This self-organized, three-dimensional, organ-oriented brain tissue model can recapitulate the process of neurogenesis and consists of diverse cell types and cellular architecture. Transplanting brain organoids in vivo could be a potential tool from bench to clinical research and has been studied for many purposes. To investigate and summarize the methodology, findings, and applications of this novel technique from current evidence, we conducted this systematic review by searching PubMed and the Embase databases for the literature ranging from 2013 to 2024. A total of 480 articles were identified, and 24 of them met the inclusion criteria. The results revealed that brain organoid transplantation had promising graft survival, neural proliferation, differentiation, and maturation, axonal growth, and functional integration into the host neuronal circuit, and has been applied to multiple applications, such as therapeutic usage, cell study platforms, and disease modeling. However, heterogeneity among studies, some significant challenges, and ethical issues remain to be considered. This comprehensive review will provide an update of what is known about this powerful, innovative method and discuss some practical aspects for future research.
近年来，脑类器官技术取得了显著进展。这种自组织的、三维的、面向器官的脑组织模型能够重现神经发生过程，由多种细胞类型和细胞结构组成。将脑类器官移植到体内可能成为从实验室研究到临床研究的一种潜在工具，并且已经针对多种目的进行了研究。为了根据现有证据研究和总结这项新技术的方法、研究结果及应用，我们通过检索PubMed和Embase数据库，对2013年至2024年的文献进行了系统综述。共识别出480篇文章，其中24篇符合纳入标准。结果显示，脑类器官移植在移植物存活、神经增殖、分化和成熟、轴突生长以及与宿主神经元回路的功能整合方面前景良好，并且已应用于多种用途，如治疗用途、细胞研究平台和疾病建模。然而，研究之间的异质性、一些重大挑战以及伦理问题仍有待考虑。这篇全面的综述将更新关于这种强大创新方法的已知信息，并讨论未来研究的一些实际问题。
Zotero 插件
