Zheng Mingming, Xiao Yunju, Zhang Wenzhe, Lv Sirui, Sun Liye, Wang Qi, Zhu Junfang, Chen Lei, Lin Hong, Luo Shihua
Department of Clinical Laboratory Medicine, Fifth People's Hospital of Jiamusi Infectious Disease Hospital, Jiamusi, Heilongjiang 154000, China.
Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510000, China.
ACS Omega. 2025 Jul 11;10(28):30651-30659. doi: 10.1021/acsomega.5c02638. eCollection 2025 Jul 22.
The detection of is crucial for the diagnosis and treatment of infectious diseases. However, developing a simple and sensitive method for detection remains challenging. Herein, we propose a sensitive surface-enhanced Raman spectroscopy (SERS) platform for the detection of using core-shell aptamer-Au@FeO nanoparticles (FeO-Au@apt) and freezing SERS tags. Core-shell Au@FeO magnetic nanoparticles were modified with specific primary aptamers for precise and efficient capture of , followed by modification with large amounts of secondary aptamers and tetramercaptobenzoic acid through freezing. The proposed SERS platform demonstrated significant sensitivity toward , with a limit of detection of 6.91 CFU/mL, which demonstrates 99.25 times higher sensitivity than conventional SERS tags. Additionally, the proposed SERS platform achieved good recovery rates in human urine and serum samples, ranging from 97.7% to 104.0% and 90.5% to 104.7%. The findings of the study suggest that the proposed SERS platform holds promise for the rapid and efficient detection of pathogenic bacteria.
对传染病的诊断和治疗而言，[病原体名称]的检测至关重要。然而，开发一种简单且灵敏的检测方法仍然具有挑战性。在此，我们提出一种灵敏的表面增强拉曼光谱（SERS）平台，用于使用核壳适配体 - 金@氧化亚铁纳米颗粒（FeO - Au@apt）和冷冻SERS标签检测[病原体名称]。核壳金@氧化亚铁磁性纳米颗粒用针对[病原体名称]的特异性初级适配体进行修饰，以精确高效地捕获[病原体名称]，随后通过冷冻用大量二级适配体和四巯基苯甲酸进行修饰。所提出的SERS平台对[病原体名称]表现出显著的灵敏度，检测限为6.91 CFU/mL，这表明其灵敏度比传统SERS标签高99.25倍。此外，所提出的SERS平台在人尿液和血清样本中实现了良好的回收率，范围分别为97.7%至104.0%和90.5%至104.7%。该研究结果表明，所提出的SERS平台有望用于快速高效地检测病原菌。
