传染性单核细胞增多症患儿血清样本中白细胞介素-10、-22、-24和-26水平升高。
Elevated interleukin-10, -22, -24, and -26 in serum samples of children with infectious mononucleosis.
作者信息
Li Dalei, Mao Kangwei, Luo Peiliang, Zheng Ziqiang, Liu Jiangyan, Zhang Chenxi, Gu Siyu, Zhang Rui, Sun Jun, Wang Juan
机构信息
The Affiliated Lianyungang Hospital of Xuzhou Medical University (the First People's Hospital of Lianyungang, the First Affiliated Hospital of Kangda College of Nanjing Medical University, Lianyungang Clinical College of Nanjing Medical University), Department of Pediatrics, Lianyungang, Jiangsu, China.
The Affiliated Lianyungang Hospital of Xuzhou Medical University (the First People's Hospital of Lianyungang, the First Affiliated Hospital of Kangda College of Nanjing Medical University, Lianyungang Clinical College of Nanjing Medical University), Department of Clinical Pharmacy, Lianyungang, Jiangsu, China.
出版信息
J Med Biochem. 2025 Jul 4;44(4):776-783. doi: 10.5937/jomb0-54625.
BACKGROUND
Infectious mononucleosis (IM) is an infectious disease with different stages of onset, and the pathogenesis of IM remains unclear. This study aimed to investigate the levels of interleukin (IL)-10 family cytokines IL-10, -22, -24, and -26 in serum samples of patients with infectious mononucleosis (IM) and the potential diagnostic values.
METHODS
Thirty children with IM were enrolled in the study, and serum samples were collected; 30 healthy children served as the controls. Serum samples from the patients and healthy controls were collected, and IL-10, -22, -24, and -26 were determined by the ELISA method. An automatic biochemical analyser detected the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Moreover, potential diagnostic values of IL-10, -22, -24 and -26 have been analysed using ROC curve analysis, and the correlation between the levels of IL-10, -22, -24 and -26 in patients was analysed. Correlation between IL-10, -22, -24, and -26 and ALT and AST were analysed.
RESULTS
IL-10, -22, -24, and -26 levels increased in serum samples of patients with IM compared to healthy controls. ROC analysis showed that the AUC of IL-10, -22, -24, and -26 were 0.8500, 0.7078, 0.8056, and 0.9167, respectively, suggesting that IL-10, -22, -24, and -26 were good biomarkers. Moreover, IL-10, -22, -24 and -26 levels were positively correlated with the ALT and AST of the patients.
CONCLUSIONS
IL-10, -22, -24, and -26 were upregulated in the serum of patients with IM, suggesting they may function as potential biomarkers.
背景
传染性单核细胞增多症（IM）是一种具有不同发病阶段的传染病，其发病机制尚不清楚。本研究旨在调查传染性单核细胞增多症（IM）患者血清样本中白细胞介素（IL）-10家族细胞因子IL-10、-22、-24和-26的水平及其潜在诊断价值。
方法
本研究纳入30例IM患儿，并采集血清样本；30例健康儿童作为对照。收集患者和健康对照的血清样本，采用ELISA法测定IL-10、-22、-24和-26。使用自动生化分析仪检测血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）。此外,采用ROC曲线分析方法分析IL-10、-22、-24和-26的潜在诊断价值，并分析患者体内IL-10、-22、-24和-26水平之间的相关性。分析IL-10、-22、-24和-26与ALT和AST之间的相关性。
结果
与健康对照相比，IM患者血清样本中IL-10、-22、-24和-26水平升高。ROC分析显示，IL-10、-22、-24和-26的AUC分别为0.8500、0.7078、0.8056和0.9167，提示IL-10、-22、-24和-26是良好的生物标志物。此外，IL-10、-22、-24和-26水平与患者的ALT和AST呈正相关。
结论
IM患者血清中IL-10、-22,-24和-26上调，提示它们可能作为潜在的生物标志物发挥作用。
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