CNU395 and CNU396 Alleviate Cognition in Scopolamine-Induced Cognitive Impairment Mice.

This study aimed to evaluate whether probiotic administration could protect against cognitive impairments in a scopolamine-induced cognitive impairment mice model. Male C57BL/6 mice (8 weeks of age) were injected with scopolamine hydrobromide to induce memory impairments. The experimental groups were additionally supplemented with 10 colony-forming units (CFU)/day probiotics containing CNU395 or CNU396. Behavioral test results and histopathological evaluations showed that the spatial memory ability and pathological tissue abnormalities of the mice in the CNU395 and CNU396 groups significantly improved compared with those in the disease group. CNU395 and CNU396 mitigated scopolamine-induced neuroinflammation by reducing the expression of pro-inflammatory cytokines (, , , and ) and the inflammasome, through the inhibition of and inflammatory pathways. Additionally, the CNU395 and CNU396 groups showed decreased levels of and , alongside increased levels of and , relative to the disease group. Therefore, CNU395 or CNU396 supplementation might help prevent the onset of cognitive deficits and neuroinflammation.