Han Dou, Xin Yu-Hua, Liu Qing
State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
College of Biological Sciences, China Agricultural University, Beijing 100093, PR China.
Int J Syst Evol Microbiol. 2025 Sep;75(9). doi: 10.1099/ijsem.0.006913.
The family , encompassing the genus and related taxa, comprises diverse Gram-negative, aerobic, rod-shaped bacteria found in varied habitats, including air, soil, water and glaciers. Recent genomic-based taxonomic revisions have reclassified some species into new genera, such as and , due to polyphyletic relationships within the family . Certain species are known for forming biofilms or functioning as aerobic anoxygenic phototrophic bacteria, traits that enhance resilience in extreme environments like the cryosphere. In this study, we isolated 12 novel strains from Tibetan Plateau glaciers, revealing significant phenotypic and genotypic diversity. Based on phylogenomic analyses, six strains were classified within , five within and one within . These strains exhibit broad pH (4-11), salt tolerance (0-3.0%) and temperature adaptability (0-37 °C), alongside varied metabolic capabilities, including diverse carbon source utilization and enzyme activities. Eleven strains exhibit biofilm formation, and some possess genes for carotenoid biosynthesis and photosynthesis. The 16S rRNA gene sequence similarities with their closest relatives ranged from 97.6% to 99.9%, while the average nt identity and digital DNA-DNA hybridization values between these strains and known species with validly published names were below 89.80% and 36.60%, respectively. Polyphasic analyses, encompassing phylogenetic, phenotypic and genotypic analyses, confirm that these strains represent 12 novel species within the family . We propose the following names: sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov. This study enhances our understanding of diversity and its ecological adaptations in extreme environments.
该菌科包含某属及相关分类群，由多种革兰氏阴性、需氧、杆状细菌组成，它们存在于包括空气、土壤、水和冰川在内的各种栖息地中。由于该菌科内的多系关系，最近基于基因组的分类学修订已将一些某属物种重新分类到新的属中，如某属和某属。某些某属物种以形成生物膜或作为需氧不产氧光合细菌发挥作用而闻名，这些特性增强了它们在冰冻圈等极端环境中的恢复力。在本研究中，我们从青藏高原冰川中分离出12株新菌株，揭示了显著的表型和基因型多样性。基于系统基因组分析，6株菌株被归类到某属内，5株在某属内，1株在某属内。这些菌株表现出广泛的pH值（4 - 11）、耐盐性（0 - 3.0%）和温度适应性（0 - 37°C），以及多样的代谢能力，包括不同碳源利用和酶活性。11株菌株表现出生物膜形成能力，有些还拥有类胡萝卜素生物合成和光合作用相关基因。它们与最亲近亲属的16S rRNA基因序列相似性在97.6%至99.9%之间，而这些菌株与有效发表名称的已知物种之间的平均核苷酸同一性和数字DNA - DNA杂交值分别低于89.80%和36.60%。包括系统发育、表型和基因型分析在内的多相分析证实，这些菌株代表了该菌科内的12个新物种。我们提议以下名称：某新种、某新种、某新种、某新种、某新种、某新种、某新种、某新种、某新种、某新种、某新种和某新种。本研究增进了我们对某菌科在极端环境中的多样性及其生态适应性的理解。
