Millman I, Scott A W, Halbherr T
Cancer Res. 1977 Nov;37(11):4150-5.
The tumor-inhibitory effect of an intralesional injection of Propionibacterium acnes was of limited duration ("finite"). Our model was the DBA/2 syngeneic mouse injected with P815 mastocytoma cells (5 X 10(5)) into each rear footpad; only the left was treated, leaving the right as a "pseudometastasis." The finite effect occurred at approximately 21 days after the first treatment. Subsequent i.p. treatments with P. acnes did not alter this effect, although they increased mean survival time. With one footpad tumor, we achieved 22% cures with complete regression and no sign of metastatic growth. A RNA fraction from P. acnes produced inhibition of tumor growth, but crude cell walls and cell walls treated with Pronase had no effect. A P. acnes cytoplasmic fraction with tumor-inhibitory activity was pelleted by high-speed centrifugation; this fraction inhibited P815 mastocytoma as fully as whole cells injected in one-fifth the dose on a nitrogen basis and did not cause a local inflammatory reaction. The activity of the pellet also differed from whole cells in that it was equally inhibitory to the pseudometastasis in the contralateral right rear footpad. The cytoplasmic fraction apparently contained at least two active components since activity was obtained at two dilution levels. Such activity was relatively stable at 5 degrees, but it was unstable at -30 degrees.
病灶内注射痤疮丙酸杆菌的肿瘤抑制作用持续时间有限（“有限”）。我们的模型是向DBA/2同基因小鼠的每个后足垫注射P815肥大细胞瘤细胞（5×10⁵）；仅对左侧进行治疗，右侧作为“假转移”对照。首次治疗后约21天出现有限效应。随后腹腔注射痤疮丙酸杆菌虽可延长平均存活时间，但并未改变这种效应。对于单足垫肿瘤，我们实现了22%的治愈率，肿瘤完全消退且无转移生长迹象。痤疮丙酸杆菌的一个RNA组分可抑制肿瘤生长，但粗制细胞壁和经链霉蛋白酶处理的细胞壁则无此作用。具有肿瘤抑制活性的痤疮丙酸杆菌细胞质组分经高速离心后形成沉淀；该组分对P815肥大细胞瘤的抑制作用与以氮计剂量为全细胞五分之一的全细胞注射效果相同，且不会引起局部炎症反应。沉淀的活性与全细胞也有所不同，它对同侧右后足垫的假转移具有同等抑制作用。细胞质组分显然至少含有两种活性成分，因为在两种稀释水平下均获得了活性。这种活性在5℃时相对稳定，但在-30℃时不稳定。
