Bissell D M, Tilles J G
J Cell Biol. 1971 Jul;50(1):222-31. doi: 10.1083/jcb.50.1.222.
A system for culturing human fetal liver cells in monolayers is described and the effects of various conditions of growth on the morphology and function of the cultured cells are presented. The addition of 10% calf serum or 1% human serum to the growth medium accelerated the proliferation of the liver cells, with subsequent loss of characteristic morphology and specific functional activity. In the absence of serum, the cultured liver cells retained their morphology and their function for at least 4 wk, as evidenced by secretion of serum albumin and storage of glycogen and iron.
本文描述了一种用于单层培养人胎儿肝细胞的系统,并展示了各种生长条件对培养细胞的形态和功能的影响。向生长培养基中添加10%的小牛血清或1%的人血清可加速肝细胞的增殖,随后细胞会丧失特征性形态和特定的功能活性。在无血清的情况下,培养的肝细胞至少4周内保持其形态和功能,血清白蛋白的分泌以及糖原和铁的储存证明了这一点。