多形核白细胞细胞膜组分的组成与性质
Composition and properties of a cell-membrane fraction from the polymorphonuclear leucocyte.
作者信息
Woodin A M, Wieneke A A
出版信息
Biochem J. 1966 May;99(2):493-500. doi: 10.1042/bj0990493.
Abstract
- The composition of a vesicular cell-membrane fraction from leucocytes has been studied. The bulk of the mass is accounted for as protein and lipid. A small amount of carbohydrate, including some N-acetylneuraminic acid, is present. The phospholipid/cholesterol molar ratio is 1.4 and differs from that for the whole cell. 2. Labile phosphorus groups are present in the membrane but the analysis is complicated by the presence of phosphorus occluded in the membrane vesicles. 3. Leucocidin does not change the gross composition of the membranes or alter the amount or reactivity of the phosphorus compounds. 4. The cell-membrane fraction has considerable avidity for an impurity present in commercial [(32)P]orthophosphate. When this is removed [(32)P]orthophosphate or [(32)P]ATP does not label the membrane. 5. The presence of an NADH(2)-cytochrome c oxidoreductase and an alkaline phosphatase is described. The adenosine-triphosphatase activity of the membrane has not been found to depend on the presence of Na(+) or K(+).
摘要
- 对白细胞来源的囊泡细胞膜组分的组成进行了研究。大部分物质为蛋白质和脂质。存在少量碳水化合物，包括一些N-乙酰神经氨酸。磷脂/胆固醇摩尔比为1.4，与全细胞的该比例不同。2. 膜中存在不稳定的磷基团，但由于膜囊泡中包埋的磷的存在，分析变得复杂。3. 白细胞杀素不会改变膜的总体组成，也不会改变磷化合物的含量或反应性。4. 细胞膜组分对市售[（32）P]正磷酸盐中存在的杂质有相当大的亲和力。去除该杂质后，[（32）P]正磷酸盐或[（32）P]ATP不会标记该膜。5. 描述了NADH（2）-细胞色素c氧化还原酶和碱性磷酸酶的存在。尚未发现膜的腺苷三磷酸酶活性依赖于Na（+）或K（+）的存在。
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