Nace C S, Szepesi B, Michaelis O E
J Nutr. 1979 Jun;109(6):1094-102. doi: 10.1093/jn/109.6.1094.
The responses of glucose-6-phosphate dehydrogenase (G6PD) (EC 1.1.1.49) and malic enzyme (ME) (EC 1.1.1.40) were studied in liver and adipose tissue of rats fed for 2 days a high glucose diet containing levels of synthetic trilinolein ranging from 0 to 25% (w/w) of the diet (trilinolein was substituted for glucose). One group of rats was starved for 2 days before the trilinolein-containing diets were fed (starved-refed); a second group of rats was fed a fat-free diet for 7 days before the trilinolein-containing diets were fed (ad libitum). Liver G6PD activity decreased exponentially and liver ME activity decreased linearly with increasing dietary trilinolein in starved-refed rats, but did not decrease significantly in ad libitum fed rats. Total liver lipid decreased exponentially with increasing trilinolein in starved-refed rats, but increased exponentially in ad libitum fed rats. Adipose tissue G6PD and ME activities decreased slightly with increasing trilinolein in starved-refed rats, but did not decrease in ad libitum fed rats. When the data were adjusted by analysis of covariance for differences in glucose intake, the liver responses in starved-refed rats were still significant but the adipose tissue responses were not, indicating that the responses of adipose tissue (but not of liver) may have resulted from decreased glucose intake rather than from increased trilinolein intake. The results suggest that dietary trilinolein inhibits the characteristic increase in liver G6PD, ME and total lipids upon starvation-refeeding. However, after the levels of these parameters have been increased by feeding a fat-free diet they cannot be decreased by dietary trilinolein in 2 days.
在以含0至25%（w/w）合成三油酸甘油酯（用三油酸甘油酯替代葡萄糖）的高糖饮食喂养2天的大鼠肝脏和脂肪组织中，研究了葡萄糖-6-磷酸脱氢酶（G6PD）（EC 1.1.1.49）和苹果酸酶（ME）（EC 1.1.1.40）的反应。一组大鼠在喂食含三油酸甘油酯的饮食之前饥饿2天（饥饿-再喂食）；另一组大鼠在喂食含三油酸甘油酯的饮食之前自由采食无脂饮食7天。在饥饿-再喂食的大鼠中，肝脏G6PD活性随饮食中三油酸甘油酯含量的增加呈指数下降，肝脏ME活性呈线性下降，但在自由采食的大鼠中没有显著下降。在饥饿-再喂食的大鼠中，肝脏总脂质随三油酸甘油酯含量的增加呈指数下降，但在自由采食的大鼠中呈指数增加。在饥饿-再喂食的大鼠中，脂肪组织G6PD和ME活性随三油酸甘油酯含量的增加略有下降，但在自由采食的大鼠中没有下降。当通过协方差分析对葡萄糖摄入量的差异进行数据调整时，饥饿-再喂食大鼠的肝脏反应仍然显著，但脂肪组织反应不显著，这表明脂肪组织（而非肝脏）的反应可能是由于葡萄糖摄入量减少而非三油酸甘油酯摄入量增加所致。结果表明，饮食中的三油酸甘油酯抑制饥饿-再喂食后肝脏G6PD、ME和总脂质的特征性增加。然而，在通过喂食无脂饮食使这些参数水平升高后，饮食中的三油酸甘油酯在2天内无法使其降低。
