DNA的核苷酸测序：鸟嘌呤、胞嘧啶或腺嘌呤残基特异性切割产物的初步表征（噬菌体M13-核糖取代-DNA聚合酶I-电泳-二维指纹图谱）
Nucleotide sequencing of DNA: preliminary characterization of the products of specific cleavages at guanine, cytosine, or adenine residues (bacteriophage M13-ribosubstitution-DNA polymerase I-electrophoresis-two-dimensional fingerprinting).
作者信息
Salser W, Fry K, Brunk C, Poon R
出版信息
Proc Natl Acad Sci U S A. 1972 Jan;69(1):238-42. doi: 10.1073/pnas.69.1.238.
Abstract
DNA synthesized in vitro from a phage M13 template has been cleaved at either guanine, adenine, or cytosine residues by ribosubstitution techniques. Fingerprints of the fragments obtained suggest that DNA sequencing will be possible with this technique.
摘要
用核糖取代技术已将从噬菌体M13模板体外合成的DNA在鸟嘌呤、腺嘌呤或胞嘧啶残基处切割。所获得片段的指纹图谱表明，用该技术进行DNA测序将是可行的。
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