大肠杆菌核糖体与N-甲酰甲硫氨酸转移RNA在起始因子IF-3依赖下与兔珠蛋白信使核糖核酸的结合
Initiation factor IF-3-dependent binding of Escherichia coli ribosomes and N-formylmethionine transfer-RNA to rabbit globin messenger.
作者信息
Noll M, Noll H, Lingrel J B
出版信息
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1843-7. doi: 10.1073/pnas.69.7.1843.
Abstract
An initiation complex has been formed in high yields from E. coli ribosomes, 9S messenger RNA for rabbit hemoglobin, and N-formylmethionine-tRNA. Initiation factor IF-3 is required for the binding and puromycin is required for the release of fMet. Valyl-tRNA fails to bind to the second codon, whereas a mixture of 15 aminoacyl-tRNAs promotes incorporation. Together with quantitative data, the findings suggest that IF-3 directs the ribosomes to an AUG codon on one of the two globin messengers, at a site that is different from the normal starting point for globin synthesis.
摘要
已从大肠杆菌核糖体、兔血红蛋白的9S信使核糖核酸和N-甲酰甲硫氨酸-转运核糖核酸高产率地形成了起始复合物。起始因子IF-3是结合所必需的，嘌呤霉素是甲酰甲硫氨酸释放所必需的。缬氨酰-转运核糖核酸不能与第二个密码子结合，而15种氨酰-转运核糖核酸的混合物则促进掺入。连同定量数据，这些发现表明IF-3将核糖体引导至两个珠蛋白信使之一上的一个AUG密码子，该位点不同于珠蛋白合成的正常起始点。
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