鼠伤寒沙门氏菌脱氧核糖核酸限制与修饰的宿主特异性
Host specificity of Salmonella typhimurium deoxyribonucleic acid restriction and modification.
作者信息
Slocum H, Boyer H W
出版信息
J Bacteriol. 1973 Feb;113(2):724-6. doi: 10.1128/jb.113.2.724-726.1973.
The restriction and modification genes of Salmonella typhimurium which lie near the thr locus were transferred to a restrictionless mutant of Escherichia coli. These genes were found to be allelic to the E. coli K, B, and A restriction and modification genes. E. coli recombinants with the restriction and modification host specificity of S. typhimurium restricted phage lambda that had been modified by each of the seven known host specificities of E. coli at efficiency of plating levels of about 10(-2). Phage lambda modified with the S. typhimurium host specificity was restricted by six of the seven E. coli host specificities but not by the RII (fi(-) R-factor controlled) host specificity. It is proposed that the restriction and modification enzymes of this S. typhimurium host specificity have two substrates, one of which is a substrate for the RII host specificity enzymes.
鼠伤寒沙门氏菌中位于苏氨酸基因座附近的限制与修饰基因被转移至大肠杆菌的一个无限制突变体中。发现这些基因与大肠杆菌K、B和A限制与修饰基因等位。具有鼠伤寒沙门氏菌限制与修饰宿主特异性的大肠杆菌重组体，能以约10⁻²的平板效率限制由大肠杆菌的七种已知宿主特异性中的每一种修饰过的噬菌体λ。用鼠伤寒沙门氏菌宿主特异性修饰的噬菌体λ受到七种大肠杆菌宿主特异性中的六种限制，但不受RII（fi⁻ R因子控制）宿主特异性限制。有人提出，这种鼠伤寒沙门氏菌宿主特异性的限制与修饰酶有两种底物，其中一种是RII宿主特异性酶的底物。
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