人初乳中一种糖蛋白的分离与鉴定
Isolation and characterization of a glycoprotein from human colostrum.
作者信息
Nichols J H, Bezkorovainy A
出版信息
Biochem J. 1973 Dec;135(4):875-80. doi: 10.1042/bj1350875.
Abstract
A glycoprotein was isolated from the M-1 acid glycoprotein fraction of human colostrum. It had a molecular weight of 31200 and contained 27% galactose, 21.7% hexosamine, 8.0% fucose and 10.8% sialic acid by weight. The glycoprotein had no absorption maxima in the 240-300nm region, and was virtually free of ABH(O) and M and N blood-group activity. Alkaline borohydride cleavage of the glycoprotein resulted predominantly in the destruction of threonine and galactosamine.
摘要
从人初乳的M-1酸性糖蛋白组分中分离出一种糖蛋白。其分子量为31200，按重量计含有27%的半乳糖、21.7%的己糖胺、8.0%的岩藻糖和10.8%的唾液酸。该糖蛋白在240 - 300nm区域没有吸收最大值，并且几乎没有ABH(O)以及M和N血型活性。该糖蛋白经碱性硼氢化物裂解主要导致苏氨酸和半乳糖胺的破坏。
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