[季也蒙毕赤酵母中核黄素生物合成第二阶段的产物]
[Product of second-stage riboflavin biosynthesis in Pichia guilliermondii].
作者信息
Logvinenko E M, Shavlovskiĭ G M, Zakal'skiĭ A E
出版信息
Mikrobiologiia. 1979 Jul-Aug;48(4):756-8.
Dialyzed extracts of the mutants of Pichia guilliermondii RG80 (rib3, his-) and RA39 (rib3, ade-) in which the third step of flavinogenesis was genetically blocked converted GTP into 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate and 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine in the presence of NADPH2. Apparently, 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate is a true precursor of riboflavin whereas 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine is formed upon its hydrolysis.
季也蒙毕赤酵母RG80（rib3，his-）和RA39（rib3，ade-）突变体的透析提取物，其中黄素生成的第三步在基因上被阻断，在NADPH2存在的情况下将GTP转化为2,5-二氨基-6-羟基-4-核醇氨基嘧啶磷酸酯和2,5-二氨基-6-羟基-4-核醇氨基嘧啶。显然，2,5-二氨基-6-羟基-4-核醇氨基嘧啶磷酸酯是核黄素的真正前体，而2,5-二氨基-6-羟基-4-核醇氨基嘧啶是在其水解时形成的。
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