通过接触杂交检测标记的RNA种类。
Detection of labelled RNA species by contact hybridization.
作者信息
Burckhardt J, Telford J, Birnstiel M L
出版信息
Nucleic Acids Res. 1979 Jul 11;6(9):2963-71. doi: 10.1093/nar/6.9.2963.
Abstract
An improved contact hybridization technique for the analysis of labelled RNA species is presented. The method combines high sensitivity of detection with the high resolution of polyacrylamide gel electrophoresis and should be especially useful for the characterization of transient RNA precursor molecules. Its application to gene mapping is illustrated.
摘要
本文介绍了一种用于分析标记RNA种类的改进型接触杂交技术。该方法将高检测灵敏度与聚丙烯酰胺凝胶电泳的高分辨率相结合，对于瞬时RNA前体分子的表征尤其有用。文中展示了其在基因图谱绘制中的应用。
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