哺乳动物细胞中的DNA复制。I. 新合成螺旋的大小。
DNA replication in mammalian cells. I. The size of newly synthesized helices.
作者信息
Nuzzo F, Brega A, Falaschi A
出版信息
Proc Natl Acad Sci U S A. 1970 Apr;65(4):1017-24. doi: 10.1073/pnas.65.4.1017.
Abstract
The newly synthesized DNA of heteroploid human cells cultured in vitro has a single-stranded molecular weight of approximately 1 x 10(5) following 30- to 60-second pulses of labeling with tritiated thymidine. With increasing pulse times the amount of radioactive DNA having a low single-stranded molecular weight remains approximately constant, whereas increasing amounts of label are found associated with the bulk DNA. After removing the label from the medium, the radioactivity associated with the small molecular weight DNA is chased into high molecular weight material. These findings are analogous to what is observed in microorganisms and suggest a basically common organization of the growing point of DNA in bacterial and in mammalian cells.
摘要
体外培养的异倍体人类细胞新合成的DNA，在用氚标记的胸腺嘧啶脉冲标记30至60秒后，其单链分子量约为1×10⁵ 。随着脉冲时间的增加，具有低单链分子量的放射性DNA量大致保持恒定，而与大量DNA相关的标记量则不断增加。从培养基中去除标记后，与小分子DNA相关的放射性被追踪到高分子量物质中。这些发现类似于在微生物中观察到的情况，表明细菌和哺乳动物细胞中DNA生长点的基本组织方式是相同的。
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