黑腹果蝇幼虫器官的大规模分离与分级分离
Large-scale isolation and fractionation of organs of Drosophila melanogaster larvae.
作者信息
Zweidler A, Cohen L H
出版信息
J Cell Biol. 1971 Oct;51(1):240-8. doi: 10.1083/jcb.51.1.240.
Abstract
Methods for the mass isolation of diverse organs from small animals are described. They involve novel devices: a mechanical dissecting system, a centrifugal agitator for the separation of fibrillar from globular particles, and a settling chamber for the fractionation at unit gravity of particles with sedimentation velocities above the useful range for centrifugation. The application of these methods to the isolation of polytene and nonpolytene nuclei from Drosophila melanogaster larvae is described.
摘要
描述了从小动物中大规模分离各种器官的方法。它们涉及新颖的装置：一种机械解剖系统、一种用于从球状颗粒中分离纤维状颗粒的离心搅拌器，以及一个沉降室，用于在单位重力下对沉降速度高于离心有用范围的颗粒进行分级分离。还描述了这些方法在从黑腹果蝇幼虫中分离多线和非多线细胞核中的应用。
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