采用33258 Hoechst-吉姆萨技术进行小鼠-人类异核体分析。
Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique.
作者信息
Moser F G, Dorman B P, Ruddle F H
出版信息
J Cell Biol. 1975 Sep;66(3):676-80. doi: 10.1083/jcb.66.3.676.
Abstract
The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.
摘要
双苯并咪唑衍生物33258 Hoechst可用于在异核体中通过显微荧光法区分小鼠和人类细胞核。这为分析此类异核体提供了一种快速且准确的放射自显影替代方法。33258 Hoechst荧光模式在照射后可转换为吉姆萨染色的差异染色呈现。
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