Hartmann K U
J Exp Med. 1971 Jun 1;133(6):1325-33. doi: 10.1084/jem.133.6.1325.
Spleen cells of bone marrow chimeras (B cells) and of irradiated mice injected with thymus cells and heterologous erythrocytes (educated T cells) were mixed and cultured together (17). The number of PFC developing in these cultures was dependent both on the concentration of the B cells and of the educated T cells. In excess of T cells the number of developing PFC is linearly dependent on the number of B cells. At high concentrations of T cells more PFC developed; the increase in the number of PFC was greatest between the 3rd and 4th day of culture. Increased numbers of educated T cells also assisted the development of PFC directed against the erythrocytes. It is concluded that the T cells not only play a role during the triggering of the precursor cells but also during the time of proliferation of the B cells; close contact between B and T cells seems to be needed to allow the positive activity of the T cells.
将骨髓嵌合体的脾细胞(B细胞)与注射了胸腺细胞和异源红细胞的受照射小鼠的脾细胞(受过“教育”的T细胞)混合并共同培养(17)。这些培养物中产生的PFC数量既取决于B细胞的浓度,也取决于受过“教育”的T细胞的浓度。在T细胞过量的情况下,发育中的PFC数量与B细胞数量呈线性相关。在高浓度T细胞时,产生的PFC更多;PFC数量的增加在培养的第3天至第4天最为显著。受过“教育”的T细胞数量增加也有助于针对红细胞的PFC的发育。结论是,T细胞不仅在祖细胞触发过程中起作用,而且在B细胞增殖期间也起作用;B细胞和T细胞之间似乎需要紧密接触才能使T细胞发挥积极作用。