巴氏芽孢杆菌不固氮的突变体。
Mutant of Clostridium pasteurianum that does not fix nitrogen.
作者信息
Simon M A, Brill W J
出版信息
J Bacteriol. 1971 Jan;105(1):65-9. doi: 10.1128/jb.105.1.65-69.1971.
Abstract
A stable, non-nitrogen-fixing mutant of Clostridium pasteurianum W5 was isolated. The mutant has the same growth rate as the wild type on ammonia nitrogen, but is not able to grow on N(2). Cell-free extracts of the derepressed mutant are unable to fix nitrogen, or reduce the alternate substrates of nitrogenase such as cyanide, azide, or acetylene. Recombination with wild-type components from a diethylaminoethyl cellulose column indicates that the mutant contains component II, but is lacking component I activity.
摘要
分离出巴氏梭菌W5的一种稳定的、不固氮的突变体。该突变体在氨氮上的生长速率与野生型相同，但不能在N₂上生长。去阻遏突变体的无细胞提取物不能固氮，也不能还原诸如氰化物、叠氮化物或乙炔等固氮酶的替代底物。与来自二乙氨基乙基纤维素柱的野生型组分重组表明，该突变体含有组分II，但缺乏组分I的活性。
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