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离体叶绿体的光合作用。卡尔文循环中间产物对正磷酸盐抑制作用的逆转。

Photosynthesis by isolated chloroplasts. Reversal of orthophosphate inhibition by Calvin-cycle intermediates.

作者信息

Cockburn W, Walker D A, Baldry C W

出版信息

Biochem J. 1968 Mar;107(1):89-95. doi: 10.1042/bj1070089.

Abstract
  1. The orthophosphate inhibition of photosynthesis by isolated spinach chloroplasts can be reversed by 3-phosphoglycerate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, fructose 6-phosphate and fructose 1,6-diphosphate. 2. Metabolically related compounds such as ribulose 1,5-diphosphate, glucose 6-phosphate, 6-phosphogluconate and phosphoenolpyruvate are ineffective. 3. The kinetics of reversal are characteristic of the intermediate used, but, in each instance, the onset of oxygen evolution is accompanied by a carbon dioxide fixation and except with 3-phosphoglycerate the stoicheiometry is close to unity. 4. The nature of orthophosphate inhibition and its reversal is discussed in relation to metabolic control of photosynthesis.
摘要
  1. 分离的菠菜叶绿体对光合作用的正磷酸盐抑制作用可被3-磷酸甘油酸、磷酸二羟丙酮、3-磷酸甘油醛、6-磷酸果糖和1,6-二磷酸果糖逆转。2. 代谢相关化合物如1,5-二磷酸核酮糖、6-磷酸葡萄糖、6-磷酸葡萄糖酸和磷酸烯醇丙酮酸无效。3. 逆转动力学是所用中间产物的特征,但在每种情况下,氧气释放的开始都伴随着二氧化碳固定,除了与3-磷酸甘油酸反应外,化学计量比接近1。4. 结合光合作用的代谢控制,讨论了正磷酸盐抑制及其逆转的性质。

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