Sanderson A R
Immunology. 1965 Sep;9(3):287-300.
A precise method has been used to investigate the kinetics and inhibition of isoimmune cytolysis of mouse lymphocytes. Target cells were labelled with Cr and lysis was estimated by measurement of the radioisotope released in a non-sedimentable form. Kinetic studies under conditions of limited complement or limited antiserum reveal a close similarity with rabbit anti-sheep haemolysis, and suggest that at least some of the molecular events causing lysis are the same in both cases. With the antisera studied, C′ probably requires a bimolecular antibody complex in order to bind to a cell, but the overall order of reaction, with respect to antiserum, may not be two. The observed variations in lytic response are believed to be due to the effects of antigen density and the presence in antisera of antibodies made of different kinds of immunoglobulins having the same or different specificities. Inhibition of lysis by syngeneic unlabelled lymphocytes is proposed as a standard in a quantitative assay for histocompatibility antigens.
一种精确的方法已被用于研究小鼠淋巴细胞同种免疫细胞溶解的动力学及抑制作用。用铬标记靶细胞，并通过测量以不可沉降形式释放的放射性同位素来估计细胞溶解情况。在补体有限或抗血清有限的条件下进行的动力学研究表明，其与兔抗羊溶血情况极为相似，这表明至少在两种情况下，导致细胞溶解的一些分子事件是相同的。在所研究的抗血清中，补体（C′）可能需要双分子抗体复合物才能与细胞结合，但就抗血清而言，反应的总体级数可能不是二级。观察到的溶解反应变化被认为是由于抗原密度的影响以及抗血清中由具有相同或不同特异性的不同种类免疫球蛋白构成的抗体的存在。同基因未标记淋巴细胞对溶解的抑制作用被提议作为组织相容性抗原定量测定的标准。
