产气荚膜梭菌（A型）细胞壁的组成成分。
Components of the cell wall of Clostridium welchii (type A).
作者信息
Pickering B T
出版信息
Biochem J. 1966 Aug;100(2):430-40. doi: 10.1042/bj1000430.
Abstract
- The cell wall of Clostridium welchii (type A) contains alanine, 2,6-diaminopimelic acid, glutamic acid, glycine, glucosamine, muramic acid, galactosamine, mannosamine, ethanolamine, rhamnose, galactose and phosphorus. 2. Heating with formamide at 150 degrees resolved the wall into a formamide-soluble polysaccharide fraction and a formamide-insoluble mucopeptide fraction. 3. The formamide-soluble fraction contained two components: an electrophoretically neutral polysaccharide made up of galactose, rhamnose, galactosamine and phosphorus and an electrophoretically acidic polymer containing mannosamine, ethanolamine and phosphorus. 4. The formamide-insoluble residue has been digested by lysozyme to give soluble fragments of high molecular weight. 5. All fractions contain an unknown ethyl acetate-extractable substance that can be oxidized by sodium metaperiodate. 6. The amino acid compositions of the fragments produced by lysozyme are compatible with a mucopeptide structure which has cross bridges containing all of the constituent amino acids.
摘要
- 魏氏梭菌（A型）的细胞壁含有丙氨酸、2,6-二氨基庚二酸、谷氨酸、甘氨酸、葡糖胺、胞壁酸、半乳糖胺、甘露糖胺、乙醇胺、鼠李糖、半乳糖和磷。2. 在150℃下用甲酰胺加热可将细胞壁分解为甲酰胺可溶性多糖部分和甲酰胺不溶性粘肽部分。3. 甲酰胺可溶性部分包含两种成分：一种由半乳糖、鼠李糖、半乳糖胺和磷组成的电泳中性多糖，以及一种含有甘露糖胺、乙醇胺和磷的电泳酸性聚合物。4. 甲酰胺不溶性残渣已被溶菌酶消化，得到高分子量的可溶性片段。5. 所有部分都含有一种未知的可被乙酸乙酯萃取的物质，该物质可被偏高碘酸钠氧化。6. 溶菌酶产生的片段的氨基酸组成与一种粘肽结构相符，该结构具有包含所有组成氨基酸的交联桥。
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