Koukol J, Dugger W M, Palmer R L
Plant Physiol. 1967 Oct;42(10):1419-22. doi: 10.1104/pp.42.10.1419.
The inhibitory effect of peroxyacetyl nitrate on the cyclic photophosphorylation of chloroplasts isolated from Black Valentine variety bean leaves (Phaseolis vulgaris L.) has been studied. Peroxyacetyl nitrate caused inhibition to photophosphorylation, in either the dark or the light, by affecting the chloroplast. Evidence is presented which suggests that peroxyacetyl nitrate could oxidize sulfhydryl groups on enzymes necessary for photophosphorylation. The inhibition to photophosphorylation caused by peroxyacetyl nitrate cannot be reversed by glutathione, even when added in large amounts, whereas the inhibition to photophosphorylation caused by para-chloromercuriphenylsulfonic acid is easily reversed by small quantities of glutathione. This suggests that if peroxyacetyl nitrate is oxidizing sulfhydryl groups necessary for photophosphorylation, this oxidation is proceeding beyond the disulfide state.
已对过氧乙酰硝酸酯对从黑瓦伦丁品种菜豆(菜豆属普通菜豆)叶片中分离出的叶绿体循环光合磷酸化的抑制作用进行了研究。过氧乙酰硝酸酯通过影响叶绿体,在黑暗或光照条件下均对光合磷酸化产生抑制作用。有证据表明,过氧乙酰硝酸酯可氧化光合磷酸化所需酶上的巯基。过氧乙酰硝酸酯对光合磷酸化的抑制作用即使大量添加谷胱甘肽也无法逆转,而对氯汞苯磺酸对光合磷酸化的抑制作用少量谷胱甘肽就能轻易逆转。这表明,如果过氧乙酰硝酸酯正在氧化光合磷酸化所需的巯基,那么这种氧化会进行到超过二硫键状态。