鲤鱼心脏中单胺氧化酶的某些特性。

Some properties of monoamine oxidase in carp heart.

作者信息

Yamamoto M, Kobayashi S, Oguchi K

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(1):117-22.

Abstract

Studies using clorgyline, deprenyl and semicarbazide as inhibitors showed that carp heart homogenate contained a new type of monoamine oxidase (MAO) and a clorgyline- and deprenyl-resistant amine oxidase (CRAO). The deamination of monoamines by carp heart MAO proceeded in two steps by a double-displacement (ping-pong) mechanism. The Km values of the MAO for oxygen (K0 values) with tyramine, 5-hydroxytryptamine (5-HT) and beta-phenylethylamine (PEA) as substrates were identical (59 microM).

摘要

使用氯吉兰、司来吉兰和氨基脲作为抑制剂的研究表明，鲤鱼心脏匀浆含有一种新型单胺氧化酶（MAO）和一种对氯吉兰和司来吉兰耐药的胺氧化酶（CRAO）。鲤鱼心脏MAO对单胺的脱氨基作用通过双置换（乒乓）机制分两步进行。以酪胺、5-羟色胺（5-HT）和β-苯乙胺（PEA）为底物时，MAO对氧的Km值（K0值）相同（59微摩尔）。