Moore G P, Costantini F D, Posakony J W, Davidson E H, Britten R J
Science. 1980 May 30;208(4447):1046-8. doi: 10.1126/science.6154974.
Cloned repetitive DNA sequences were used to determine the number of homologous RNA transcripts in the eggs of two sea urchin species, Strongylocentrotus purpuratus and S. franciscanus. The eggs of these species contain different amounts of RNA, and their genomes contain different numbers of copies of the cloned repeats. The specific pattern of repetitive sequence representation in the two egg RNA's is nonetheless quantitatively similar. The evolutionary conservation of this pattern suggests the functional importance of repeat sequence expression.
克隆的重复DNA序列被用于确定两种海胆——紫海胆和加州海胆卵子中同源RNA转录本的数量。这些物种的卵子含有不同量的RNA,并且它们的基因组含有不同数量的克隆重复序列拷贝。然而,两种卵子RNA中重复序列呈现的特定模式在数量上是相似的。这种模式的进化保守性表明重复序列表达具有功能重要性。