一种用于研究甲型流感病毒与人类红细胞膜相互作用及融合的“网格上”电子显微镜方法。

An 'on grid' electron microscopic method for studying the interaction and fusion of influenza A virus with human erythrocyte membranes.

作者信息

Fidgen K J, Tisdale M

出版信息

J Virol Methods. 1981 Dec;3(5):271-6. doi: 10.1016/0166-0934(81)90064-1.

Abstract

An 'on grid' negative staining method was developed to study the interaction of influenza virus with erythrocyte membranes. The virus was reacted in situ with cells which had been immobilised on an electron microscope grid. Fusion between influenza virus and the membrane of erythrocyte ghosts was clearly seen using this method.

摘要

开发了一种“在网格上”的负染色方法来研究流感病毒与红细胞膜的相互作用。病毒在原位与固定在电子显微镜网格上的细胞发生反应。使用这种方法可以清楚地看到流感病毒与红细胞血影膜之间的融合。