4'-(羟甲基)-4,5',8-三甲基补骨脂素光加成反应对核糖核酸构象的依赖性

Dependence of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen photoaddition on the conformation of ribonucleic acid.

作者信息

Thompson J F, Bachellerie J P, Hall K, Hearst J E

出版信息

Biochemistry. 1982 Mar 16;21(6):1363-8. doi: 10.1021/bi00535a040.

Abstract

The photoaddition of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A) . poly(U) (double stranded), and poly(U) . poly(A) . poly(U) (triple stranded) were reacted with HMT at different temperatures and salt concentrations. The conformation of the polymers was monitored by UV absorption and circular dichroism. It was found that the rate of HMT photoaddition changed dramatically at structural transitions in the RNA. The alternating copolymer poly(A-U) was found to have the highest rate of addition. Low salt and temperature produced maximal incorporation.

摘要

研究了4'-(羟甲基)-4,5',8-三甲基补骨脂素（HMT）与RNA不同构象状态的光加成反应。聚尿苷酸（Poly(U)）、聚腺苷酸-尿苷酸（Poly(A,U)，无规共聚物）、聚（A-U）（交替共聚物）、聚腺苷酸·聚尿苷酸（Poly(A). Poly(U)，双链）和聚尿苷酸·聚腺苷酸·聚尿苷酸（Poly(U). Poly(A). Poly(U)，三链）在不同温度和盐浓度下与HMT反应。通过紫外吸收和圆二色性监测聚合物的构象。结果发现，在RNA的结构转变处，HMT光加成的速率发生了显著变化。发现交替共聚物聚（A-U）具有最高的加成速率。低盐和低温产生最大掺入量。